Virtual simulation (VS) is a hot issue in the field of higher education. At present, VS has been applied to experimental teaching in universities. In order to promote the progress of VS teaching of experimental animal science, this paper summarizes the characteristics, defects and deficiencies of existing software courses in the market, and introduces a variety of simulation objects of VS courses of medical experimental animal science. It includes the audience of the course and job roles, model animals and disease models, and laboratory facilities and equipment and instrument parameters. At the same time, this paper also discusses the specific content of VS used for teaching curriculum, such as animal breeding and feeding, infected and non-infected animal experiments, facility and equipment overhaul and maintenance, animal experiment safety and admission training, etc., and gives professional advice on updating and optimizing the VS curriculum.

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