Objective To characterize the disease burden and trends of road injury-related traumatic brain injury (RI-TBI) in China from 1990 to 2021.Methods Using data from the Global Burden of Disease Study 2021, the RI-TBI incidence, prevalence, and years lived with disability (YLD) for China and its 33 provincial-level administrative divisions between 1990 and 2021 were estimated. Temporal trends were eva

Objective To establish a stably passaged tree shrew aortic endothelial cell line (TSAEC), and to explore its application in respiratory syncytial virus (RSV) infection research.Methods Primary aortic endothelial cells derived from tree shrews were isolated and cultured using the tissue explant adherence method, and an immortalized TSAEC was established through lentivirus-mediated SV40T gene transd

Objective To investigate the current status of evidence grading and/or recommendation classification in clinical practice guidelines for type 2 diabetes mellitus (T2DM) in China, and to provide references for improving the methodological quality of domestic diabetes guidelines.Methods The publicly released clinical practice guidelines/consensus(hereofter referred to as the guidelines) for adult T2

Objective　To investigate the association between triglyceride-glucose-body mass index (TyG-BMI) and depressive symptoms in individuals with cardiovascular-kidney-metabolic (CKM) syndrome, and its heterogeneity across different stages.Methods This study utilized data from a survey on comorbidities among adults in rural communities in Yichang of Hubei province, conducted in August 2023. Participant

Nested case-control study (NCCS) is a derived type of traditional case-control study. As an observational research method, it has many advantages and provides an efficient, economic and reliable method for clinical research. This article elaborates on the progress of NCCS and their applications in clinical research from the perspective of clinical epidemiological methodology. The principles, class