Objective  To investigate the effects of micro RNA-222-3p (miR-222-3p) on the proliferation, apoptosis and invasion of oral squamous cell carcinoma (OSCC) CAL-27 cells.

Methods  qRT-PCR was used to detect the expression of miR-222-3p in CAL-27 cells and normal oral keratinocyte cell line (hNOK). CAL-27 cells were cultured and divided into control group (NC group), inhibitor NC group (transfected negative control plasmid ) and miR-222-3p inhibitor group (transfected into miR-222-3p inhibitor). MTT, flow cytometry and Trasnwell method were used to detect the changes of cell proliferation, apoptosis and invasion ability, respectively, and Western Blot was used to detect the expression of BAX, Bcl-2, MMP-2, N-cadherin and E-cadherin.

Results  The expression of miR-222-3p in CAL-27 cells was significantly higher than that of hNOK cells (P<0.05). Compared with inhibitor NC group and NC group, the apoptosis rate was significantly increased, and the proliferation and invasion ability of CAL-27 cells was significantly decreased (P<0.05), the expression levels of Bcl-2, MMP-2 and N-cadherin were significantly decreased, while the expression levels of BAX and E-cadherin were significantly increased in miR-222-3p inhibitor group (P<0.05).

Conclusion  miR-222-3p is highly expressed in CAL-27 cells, and down-regulation of miR-222-3p can inhibit the proliferation and invasion of CAL-27 cells, and promote cell apoptosis

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